Protein isoprenylation in suspension-cultured tobacco cells.
نویسندگان
چکیده
منابع مشابه
A plastidial pathway for protein isoprenylation in tobacco cells.
Isoprenylation is an important posttranslational modification of proteins in eukaryotic cells, as it facilitates protein–membrane and protein–protein interactions. In plants, isoprenylated proteins (which include a number of small GTPases and the G-protein g-subunit) are involved in phytohormone signaling, meristem maintenance, cell cycle regulation, and stress responses (Crowell, 2000; Johnson...
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Isoprenylation is a posttranslational modification that is believed to be necessary, but not sufficient, for the efficient association of numerous eukaryotic cell proteins with membranes. Additional modifications have been shown to be required for proper intracellular targeting and function of certain isoprenylated proteins in mammalian and yeast cells. Although protein isoprenylation has been ...
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Soybean cells in suspension culture incorporate [(3)H]mannose into dolichyl-phosphoryl-mannose and into lipid-linked oligosaccharides as well as into extracellular and cell wall macromolecules. Tunicamycin completely inhibited the formation of lipid-linked oligosaccharides at a concentration of 5 to 10 micrograms per milliliter, but it had no effect on the formation of dolichyl-phosphoryl-manno...
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The transfer of a methyl group from S-adenosyl-L-methionine onto the carboxyl group of alpha-1,4-linked-galactosyluronic acid residues in the pectic polysaccharide homogalacturonan (HGA) is catalyzed by an enzyme commonly referred to as pectin methyltransferase. A pectin methyltransferase from microsomal membranes of tobacco (Nicotiana tabacum) was previously characterized (F. Goubet, L.N. Coun...
متن کاملStructures of N-linked oligosaccharides of glycoproteins from tobacco BY2 suspension cultured cells.
The structures of N-linked sugar chains of glycoproteins expressed in tobacco BY2 cultured cells are reported. Five pyridylaminated (PA-) N-linked sugar chains were derived and purified from hydrazinolysates of the glycoproteins by reversed-phase HPLC and size-fractionation HPLC. The structures of the PA-sugar chains purified were identified by two-dimensional PA-sugar chain mapping, ion-spray ...
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ژورنال
عنوان ژورنال: The Plant Cell
سال: 1993
ISSN: 1040-4651,1532-298X
DOI: 10.1105/tpc.5.4.433